МРНТИ 34.15.31                                                                                          №4 (2020г.)

Seidalina A.B., Kydyrmanov A.I., Karamendin K.O., Kassymbekov Ye.T., Khan E.Ya., Daulbaeva K.D., Suleimenova S.A., Yessentureyeva M.O., Sayatov   M.Kh.

In October 2013, an infectious agent with a hemagglutinating titer of 1: 512 and an with infectiousness of 7,6 lg EID50 / 0.2 ml was isolated from the
cloacal swab of a white-fronted goose in the lake system of the North Kazakhstan region Kazakhstan. The identification of the hemagglutinating agent in PCR with primers for the conserved regions of the L gene allowed us to attribute it to the Paramyxoviridae family. The method of sequencing a fragment of the L-gene and subsequent BLAST analysis showed the circulation of a novel previously unknown Avian orthoavulavirus 13 (APMV-13) in populations of wild birds of Kazakhstan. We carried out genome sequencing of the Kazakh isolate APMV-13/ white-fronted goose / Northern Kazakhstan / 5751/2013. The sequence of genes was determined: 3’-NP-P/ V / W-M-F-HN-L-5 ’, which encodes eight proteins distinctive to
Avian orthoavulavirus. Phylogenetic studies have shown that the Kazakh Avian orthoavulavirus 13 (APMV-13) is a novel natural variant, significantly different from other species.
Keywords: strain, paramyxovirus, seratype, population, wild birds, PCR.